Figure 5.
Effects of WOX4 Overexpression on Vascular Development and Root Growth.
(A) Relative expression level of WOX4 per TUA4 in the 35Spro:WOX4 lines measured by qRT-PCR. The wild-type expression level is set to 1.
(B) Stele width of 7-d-old hypocotyls. No significant differences were found in the four different 35Spro:WOX4 lines when compared with the wild type (Col-0).
(C) and (D) Transverse sections of 7-d-old hypocotyls. Cellular arrangement in the stele was similar between the wild type (C) and 35Spro:WOX4 (D).
(E) Relative expression of TDIF genes (CLE41 and CLE44) per TUA4 in 35Spro:WOX4 lines (#6 and #18). The wild-type expression level is set to 1.
(F) Root growth of 7-d-old plants.
(G) Tissue anatomy in the RAM of 7-d-old plants.
Bars = 20 μm in (C) and (D), 1 cm in (F), and 100 μm in (G). Error bars indicate sd (n = 3, *Student’s t test significance at P < 0.05 for different means compared with the wild-type expression levels) in (A) and (E) and se (n = 9 to 10, *Student’s t test significance at P < 0.01 for different means compared with the wild type) in (B).