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Determination of Cys Modifications of TGA1
| 10 μM GSNO |
0.1 mM GSNO |
0.5 mM GSNO |
||||
| S-NO | S-SG | S-NO | S-SG | S-NO | S-SG | |
| C172 | − | + | − | + | + | + |
| C260 | + | + | + | + | + | + |
| C266 | + | + | + | + | + | + |
| C287 | − | − | − | − | + | + |
Purified recombinant TGA1 was treated with different concentrations of GSNO for 10 min. Residual GSNO was removed by gel filtration. After tryptic digestion, the Cys-containing peptides were analyzed for their modification by nano-liquid chromatography tandem mass spectrometry.
