Figure 1.

VLN5 Is Expressed Preferentially in Pollen.

The expression pattern of VLN5 throughout the Arabidopsis plant was examined by RT-PCR of separated tissues and with a promoter-reporter fusion.

(A) VLN5-specific primers were used to determine tissue expression patterns by RT-PCR. Tubulin2 was used as an internal loading control. The samples are as follows: lane 1, seedling; lane 2, root; lane 3, internode; lane 4, bottom part of stem; lane 5, juvenile leaf; lane 6, adult leaf; lane 7, flower; lane 8, anther; lane 9, pollen; lane 10, silique at early stage; lane 11, silique at late stage.

(B) VLN5 promoter activity was determined using GUS as a reporter. Samples include (a) 7-d-old seedling, (b) leaves, (c) stems, (d) flower, and (e) siliques at different stages. Inset: pollen grain and germinated tube. Bar = 10 μm for the pollen tube.