Figure 3.

VLN5 Loss-of-Function Mutant Plants Have Retarded Pollen Tube Growth.

(A) to (C) Micrographs of pollen tubes after germination for 2 h in vitro. Pollen was isolated from plants with the following genotypes: wild-type Col-0 (A), homozygous vln5-1 (B), and homozygous vln5-2 (C). Bar in (C) = 100 μm.

(D) to (F) Length distribution of pollen tubes: wild-type Col-0 (D), vln5-1 (E), and vln5-2 (F).

(G) Measurement of growth rates was performed by tracking individual pollen tubes. (a) to (f) Pairs of images from single pollen tubes selected for measurement: (a) wild-type Col-0 pollen tube and (b) the same pollen tube after 30 min of growth; (c) vln5-1 pollen tube and (d) the same pollen tube after 30 min growth; (e) vln5-2 pollen tube and (f) the same pollen tube after 30 min growth. Bar = 50 μm in (f) for (a) to (f). (g) A plot of pollen tube growth rates shows that vln5 mutant pollen tubes had reduced rates compared with the wild type. Wild-type Col-0, black bar; vln5-1, gray bar; vln5-2, white bar. Error bars represent mean values ± se; n = 200. Pollen tube growth rate of vln5 pollen tubes was significantly different from that of wild-type Col-0 pollen tubes as determined by analysis of variance followed by Dunnett post-hoc multiple comparisons; **P < 0.01.