Figure 4.

VLN5 Loss of Function Does Not Affect the Amount or Distribution of Actin Filaments in Ungerminated Pollen Grains and Pollen Tubes.

Pollen grains and pollen tubes from wild-type Col-0 and VLN5 loss-of-function mutants were subjected to actin filament staining with Alexa-488 phalloidin. More than 50 stained pollen tubes were obtained for each genotype, and typical images for each genotype, which represented >85% of the population, are presented. See Supplemental Figure 7 for more examples. The images of actin staining of pollen grains and pollen tubes are projections of confocal optical sections. Bar = 7.5 μm in (I) for pollen grains and 10 μm in (J) for pollen tubes.

(A) Wild-type Col-0 pollen grain.

(B) Wild-type Col-0 pollen tube. On the right is a transmitted light image of the corresponding pollen tube.

(C) vln5-1 pollen grain.

(D) vln5-1 pollen tube and corresponding transmitted light image.

(E) vln5-2 pollen grain.

(F) vln5-2 pollen tube and corresponding transmitted light image.

(G) VLN5 RNAi line 1 pollen grain.

(H) VLN5 RNAi line 1 pollen tube and corresponding transmitted light image.

(I) VLN5 RNAi line 2 pollen grain.

(J) VLN5 RNAi line 2 pollen tube and corresponding transmitted light image.