Figure 6.

Pollen Grains and Pollen Tubes with Reduced VLN5 Levels Have Unstable Actin Filaments.

Ungerminated pollen grains and pollen tubes treated with 100 nM LatB for 30 min were subjected to actin staining with Alexa-488 phalloidin. Transmitted light images of each pollen tube are shown to the right of the fluorescence microscope image.

(A) Wild-type Col-0 pollen grain.

(B) Wild-type Col-0 pollen tube.

(C) vln5-1 pollen grain.

(D) vln5-1 pollen tube.

(E) vln5-2 pollen grain.

(F) vln5-2 pollen tube. Bar = 7.5 μm in (E) for pollen grains and 10 μm in (F) for pollen tubes.

(G) Quantification of relative F-actin level in pollen grains treated with 100 nM LatB. The amount of F-actin in treated wild-type Col-0 pollen grains was normalized to 100%. Error bars represent ± sd (n = 10); **P < 0.01 (Student’s t test).

(H) Quantification of relative F-actin level in pollen tubes treated with 100 nM LatB. The amount of F-actin in treated wild-type Col-0 pollen tubes was normalized to 100%. Error bars represent ± sd (n > 27); *P < 0.05 (Student’s t test).