Table 2.
No. of Correlations to Other Enzymes |
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Enzymes Individual Enzymes | Rs, Enzyme Activity versus Total Protein | pBH < 0.01 | pBH < 0.05 | Specific Activitya | Average Maximum Activity | Average Amount as % of Total Protein | Rs (% Protein in Enzyme versus % Protein in All-Rubisco) | Rs (% Protein in Enzyme versus % Protein in All Enzymes) |
Rubisco | 0.32 | 13 | 18 | 1,600 | 8,268 | 28.38 | 0.491 | 0.964 |
PGK | 0.24 | 10 | 16 | 983,684 | 77,735 | 0.45 | 0.548 | 0.405 |
NADP-GAPDH | 0.16 | 15 | 19 | 80,000 | 10,852 | 0.78 | 0.703 | 0.453 |
TPI | 0.40 | 17 | 23 | 6,858,936 | 155,855 | 0.13 | 0.663 | 0.500 |
TK | 0.16 | 7 | 16 | 22,000 | 8,402 | 2.08 | 0.698 | 0.436 |
Glycerate kinase | 0.27 | 13 | 19 | 54,600 | 2,607 | 0.26 | 0.474 | 0.339 |
NADP-MDH | 0.20 | 16 | 19 | 32,527 | 1,159 | 0.19 | 0.498 | 0.336 |
cFBP* | 0.21 | 0 | 2 | 44,194 | 111 | 0.00 | 0.184 | 0.220 |
cPGI | 0.25 | 6 | 17 | 495,000 | 1,836 | 0.00 | 0.442 | 0.392 |
PGM | 0.41 | 8 | 16 | 82,500 | 4,042 | 0.26 | 0.415 | 0.489 |
UGP* | 0.20 | 3 | 12 | 435,058 | 4,095 | 0.06 | 0.386 | 0.376 |
SPS* | −0.06 | 4 | 9 | 44,750 | 510 | 0.06 | 0.366 | 0.317 |
AGP | 0.24 | 12 | 24 | 39,175 | 700 | 0.13 | 0.454 | 0.373 |
pPGI | 0.33 | 9 | 18 | 495,000 | 1,192 | 0.00 | 0.423 | 0.325 |
PFK | 0.11 | 3 | 10 | 219,380 | 176 | 0.00 | 0.312 | 0.133 |
PFP | 0.09 | 1 | 4 | 23,150 | 161 | 0.06 | 0.363 | 0.212 |
NAD-GAPDH | 0.17 | 9 | 17 | 105,000 | 16,756 | 0.91 | 0.672 | 0.498 |
PK | 0.25 | 12 | 18 | 88,388 | 1,006 | 0.06 | 0.440 | 0.190 |
PEPC | −0.03 | 10 | 17 | 30,653 | 744 | 0.13 | 0.549 | 0.401 |
NAD-MDH | 0.28 | 18 | 24 | 800,000 | 53,492 | 0.39 | 0.639 | 0.486 |
Fumarase | 0.00 | 2 | 3 | 293,012 | 4,423 | 0.06 | 0.226 | 0.114 |
NADP-ICDH | 0.17 | 6 | 12 | 24,254 | 1,466 | 0.32 | 0.141 | 0.133 |
CS | 0.01 | 2 | 3 | n.a. | 190 | n.a. | n.a. | n.a. |
NAD-ICDH* | 0.07 | 3 | 6 | 11,000 | 55 | 0.00 | 0.225 | 0.025 |
NR* | 0.13 | 1 | 1 | 55,000 | 409 | 0.06 | 0.160 | 0.222 |
GS | 0.20 | 9 | 16 | 22,627 | 1,148 | 0.26 | 0.476 | 0.292 |
GOGAT | 0.20 | 0 | 0 | 40,800 | 1,803 | 0.26 | 0.099 | 0.195 |
AlaAT | 0.24 | 11 | 18 | 45,573 | 5,942 | 0.71 | 0.601 | 0.441 |
AspAT | 0.37 | 11 | 16 | 420,000 | 6,236 | 0.06 | 0.461 | 0.246 |
Shikimate DH | 0.23 | 12 | 17 | 86,000 | 600 | 0.06 | 0.480 | 0.430 |
GLDH | −0.01 | 4 | 14 | 62,680 | 576 | 0.06 | 0.483 | 0.210 |
G6PDH* | 0.04 | 0 | 2 | 62,075 | 268 | 0.00 | 0.243 | 0.130 |
Susy* | 0.05 | 0 | 3 | 180,000 | 122 | 0.00 | 0.194 | 0.190 |
INV | −0.04 | 0 | 1 | 115,566 | 1,223 | 0.06 | 0.227 | 0.260 |
FK | 0.09 | 4 | 9 | 10,500 | 117 | 0.06 | 0.270 | 0.247 |
GK | 0.13 | 3 | 7 | 43,240 | 112 | 0.00 | 0.262 | 0.326 |
Grouped in pathways | 0.00 | |||||||
All enzymes Rubisco | 6.8 | 12.2 | 7.99 | 1.000 | 0.672 | |||
All enzymes | 7.0 | 12.3 | 36.36 | 0.672 | 1.000 | |||
Calvin-Benson cycle | 13.7 | 18.7 | 31.82 | 0.606 | 0.991 | |||
Calvin-Benson cycle minus Rubisco | 13.2 | 18.2 | 3.44 | 0.870 | 0.553 | |||
Sucrose synthesis | 6.0 | 13.0 | 0.39 | 0.583 | 0.586 | |||
Starch synthesis | 12.5 | 23.0 | 0.13 | 0.493 | 0.405 | |||
Respiration | 9 | 13.6 | 1.95 | 0.805 | 0.580 | |||
Amino acid metabolism | 11.3 | 16.3 | 1.43 | 0.645 | 0.487 | |||
Sucrose breakdown | 6.3 | 9.5 | 0.13 | 0.364 | 0.367 |
The Rs values for correlations between enzyme activity (nmol/min·gFW) and protein content (mg/gFW) and the number of correlations between enzyme pairs are from Supplemental Data Set 2A online. The measured maximum enzyme activities (nmol/min·g FW; from Supplemental Data Set 1E online) were divided by literature values for specific activities (a; nmol/min.mg protein; see Piques et al., 2009 for sources) to estimate the amount of protein invested in each enzyme (mg protein/gFW). The average values for a given enzyme across all accessions are shown. The individual values for each enzyme were summed in each accession and used to calculate the total amount of protein invested in enzymes in that accession. The Rs values are given for the regression plot between the amount of protein in a given enzyme and the summed estimate of protein in all enzymes and for the corresponding plot but omitting Rubisco from the sum of protein. Rs values are coded according to significance (italic and bold for pBH < 0.05 and 0.01, respectively). *enzyme determined in 62 to 92 accessions over 129. n.a., not available.
In nmol/min·mg−1 purified enzyme.