. 2010 Aug 10;22(8):2872–2893. doi: 10.1105/tpc.110.076653

Table 2.

Connectivities of Enzyme Activities

		No. of Correlations to Other Enzymes
Enzymes Individual Enzymes	R_s, Enzyme Activity versus Total Protein	p_BH < 0.01	p_BH < 0.05	Specific Activity^a	Average Maximum Activity	Average Amount as % of Total Protein	R_s (% Protein in Enzyme versus % Protein in All-Rubisco)	R_s (% Protein in Enzyme versus % Protein in All Enzymes)
Rubisco	0.32	13	18	1,600	8,268	28.38	0.491	0.964
PGK	0.24	10	16	983,684	77,735	0.45	0.548	0.405
NADP-GAPDH	0.16	15	19	80,000	10,852	0.78	0.703	0.453
TPI	0.40	17	23	6,858,936	155,855	0.13	0.663	0.500
TK	0.16	7	16	22,000	8,402	2.08	0.698	0.436
Glycerate kinase	0.27	13	19	54,600	2,607	0.26	0.474	0.339
NADP-MDH	0.20	16	19	32,527	1,159	0.19	0.498	0.336
cFBP*	0.21	0	2	44,194	111	0.00	0.184	0.220
cPGI	0.25	6	17	495,000	1,836	0.00	0.442	0.392
PGM	0.41	8	16	82,500	4,042	0.26	0.415	0.489
UGP*	0.20	3	12	435,058	4,095	0.06	0.386	0.376
SPS*	−0.06	4	9	44,750	510	0.06	0.366	0.317
AGP	0.24	12	24	39,175	700	0.13	0.454	0.373
pPGI	0.33	9	18	495,000	1,192	0.00	0.423	0.325
PFK	0.11	3	10	219,380	176	0.00	0.312	0.133
PFP	0.09	1	4	23,150	161	0.06	0.363	0.212
NAD-GAPDH	0.17	9	17	105,000	16,756	0.91	0.672	0.498
PK	0.25	12	18	88,388	1,006	0.06	0.440	0.190
PEPC	−0.03	10	17	30,653	744	0.13	0.549	0.401
NAD-MDH	0.28	18	24	800,000	53,492	0.39	0.639	0.486
Fumarase	0.00	2	3	293,012	4,423	0.06	0.226	0.114
NADP-ICDH	0.17	6	12	24,254	1,466	0.32	0.141	0.133
CS	0.01	2	3	n.a.	190	n.a.	n.a.	n.a.
NAD-ICDH*	0.07	3	6	11,000	55	0.00	0.225	0.025
NR*	0.13	1	1	55,000	409	0.06	0.160	0.222
GS	0.20	9	16	22,627	1,148	0.26	0.476	0.292
GOGAT	0.20	0	0	40,800	1,803	0.26	0.099	0.195
AlaAT	0.24	11	18	45,573	5,942	0.71	0.601	0.441
AspAT	0.37	11	16	420,000	6,236	0.06	0.461	0.246
Shikimate DH	0.23	12	17	86,000	600	0.06	0.480	0.430
GLDH	−0.01	4	14	62,680	576	0.06	0.483	0.210
G6PDH*	0.04	0	2	62,075	268	0.00	0.243	0.130
Susy*	0.05	0	3	180,000	122	0.00	0.194	0.190
INV	−0.04	0	1	115,566	1,223	0.06	0.227	0.260
FK	0.09	4	9	10,500	117	0.06	0.270	0.247
GK	0.13	3	7	43,240	112	0.00	0.262	0.326
Grouped in pathways						0.00
All enzymes Rubisco		6.8	12.2			7.99	1.000	0.672
All enzymes		7.0	12.3			36.36	0.672	1.000
Calvin-Benson cycle		13.7	18.7			31.82	0.606	0.991
Calvin-Benson cycle minus Rubisco		13.2	18.2			3.44	0.870	0.553
Sucrose synthesis		6.0	13.0			0.39	0.583	0.586
Starch synthesis		12.5	23.0			0.13	0.493	0.405
Respiration		9	13.6			1.95	0.805	0.580
Amino acid metabolism		11.3	16.3			1.43	0.645	0.487
Sucrose breakdown		6.3	9.5			0.13	0.364	0.367

The R_s values for correlations between enzyme activity (nmol/min·gFW) and protein content (mg/gFW) and the number of correlations between enzyme pairs are from Supplemental Data Set 2A online. The measured maximum enzyme activities (nmol/min·g FW; from Supplemental Data Set 1E online) were divided by literature values for specific activities (a; nmol/min.mg protein; see Piques et al., 2009 for sources) to estimate the amount of protein invested in each enzyme (mg protein/gFW). The average values for a given enzyme across all accessions are shown. The individual values for each enzyme were summed in each accession and used to calculate the total amount of protein invested in enzymes in that accession. The R_s values are given for the regression plot between the amount of protein in a given enzyme and the summed estimate of protein in all enzymes and for the corresponding plot but omitting Rubisco from the sum of protein. R_s values are coded according to significance (italic and bold for p_BH < 0.05 and 0.01, respectively). *enzyme determined in 62 to 92 accessions over 129. n.a., not available.

In nmol/min·mg⁻¹ purified enzyme.