Table 1.
Disturbed Auxin Levels in UGT74E2-Overexpressing Plants
| Auxins | Wild Type | UGTOE3.10 | UGTOE13.8 |
| Experiment 1: Control | |||
| IBAa | 0.48 ± 0.14 | 1.03 ± 0.27 | 1.37 ± 0.33 |
| IBA-Glca | 1,980 ± 330 | 3,287 ± 462 | 5,196 ± 768 |
| IAA | 88.98 ± 18.07 | 82.75 ± 8.76 | 88.60 ± 5.73 |
| IAA-Glc | 304.59 ± 29.45 | 193.51 ± 30.04 | 224.24 ± 6.27 |
| oxIAA | 305.29 ± 27.91 | 370.39 ± 52.50 | 407.48 ± 18.79 |
| IAA-Glu | 13.94 ± 3.00 | 19.65 ± 4.51 | 21.68 ± 4.48 |
| IAA-Asp | 6.22 ± 1.32 | 6.83 ± 1.40 | 7.89 ± 1.24 |
| IAA-Ala | 5.01 ± 1.61 | 4.06 ± 0.74 | 5.21 ± 0.57 |
| metIAA | 85.79 ± 12.48 | 82.97 ± 8.65 | 99.52 ± 4.99 |
| Experiment 2: PEG | |||
| IBA | 5.67 ± 0.24 | 5.95 ± 2.05 | 7.33 ± 1.89 |
| IBA-Glca | 21,002.09 ± 4,319.61 | 29,964.16 ± 15,223.65 | 31,770.65 ± 4,721.77 |
| IAA | 58.21 ± 4.03 | 97.68 ± 2.69 | 111.47 ± 7.97 |
| IAA-Glca | 2,081.62 ± 358.24 | 1,233.26 ± 114.94 | 1,003.56 ± 71.46 |
Measurements of endogenous auxin content (pmol g−1 tissue fresh weight) under control or PEG-induced stress conditions. Five replicates were tested statistically by ANOVA. Data are means ± se. Statistically significant differences are indicated in bold (n = 5, P < 0.05).
a
For experiment 1, IBA and IBA-Glc measurements (n = 9 to 10) were P = 0.06 for IBA and P = 0.004 for IBA-Glc. Hormone levels for wild-type and transgenic lines grown under control conditions for experiment 2 were of similar magnitude as observed in experiment 1; P = 5 × 10−4 for IBA-Glc and P = 9 × 10−6 for IAA-Glc.