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. 2010 Aug 10;22(8):2642–2659. doi: 10.1105/tpc.109.071720

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© 2010 American Society of Plant Biologists

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Figure 11. — Addition of 8:0/8:0 DAG or 8:0/8:0 PA Restored npc4-1 Seedlings’ ABA Sensitivity to the Wild Type.

(A) Seedling morphology and root growth of npc4-1, OE, COM, and the wild type in the presence of 20 μM ABA without or with 8:0/8:0 DAG, 8:0/8:0 PA, and/or DGKI1. Four-day-old seedlings were transferred to 0.5× Murashige and Skoog medium with or without 20 μM 8:0/8:0 DAG, 8:0/8:0 PA, and DGKI1 and grown for 10 d. COM, the wild type complemented with NPC4. Bar = 1.3 cm.

(B) Root length of NPC4-KO, OE, COM, and the wild type in response to 20 μM ABA in the presence of 8:0/8:0 DAG, 8:0/8:0 PA, 8:0/8:0 PG, 8:0/8:0 PC, and/or DGKI1. Values are means ± sd (n = 10). *Significant at P < 0.05 compared with the wild type based on Student’s t test. Experiments were performed at least three times with similar results, and the values presented represent one experiment. COM, the wild type complemented with NPC4. Lipid abbreviations are described in the legend to Figure 5. DAGKI1, DAG kinase inhibitor 1.