Figure 8.

Opposite Effects of npc4-1 and NPC4-OE on Tolerance to Salt Stress.

(A) Quantification of total germination and viable growth of wild-type, npc4-1, and NPC4-OE seedlings on 0.5× Murashige and Skoog medium plus 200 mM NaCl for 2 weeks. Total germination represents the percentage of radicle emergence, and viable seedlings refer to seedlings not bleached and remaining green. One hundred seeds per genotype were germinated. Values are means ± sd; n = 3. *Significant at P < 0.05 compared with the wild type based on Student’s t test.

(B) Seedling phenotypes under salt stress. Five-day-old seedlings were transferred to 0.5× Murashige and Skoog medium with and without NaCl. Photographs were taken 10 d after transfer. Bar = 1.3 cm.

(C) Root length of wild-type, npc4-1, and OE seedling grown on 0.5× Murashige and Skoog medium plus 0, 100, or 150 mM NaCl for 10 d. Values are means ± sd (n = 15).

(D) Biomass production under salt stress. The rosettes from the wild type, KO, and OE were harvested on day 10 from plants subjected to salt stress as described in (C) and oven dried for 2 d at 60°C. Values are means ± sd (n = 15). Three independent experiments were performed with similar results, and the values of one experiment are presented.