Figure 5.

Cross Sections of Differently Affected Pollen Grains of Heterozygous eno1 Mutants in the Homozygous cue1 Background (ccEe) Analyzed by Transmission Electron Microscopy in the Tricellular Stage in Comparison to Wild-Type Col-0.

(A) Cross section of a wild-type (Col-0) pollen grain.

(B) Close-up of the wild-type pollen grain shown in (A).

(C) to (L) Phenotypic changes in the ultrastructure of pollen grains observed in pollen sacs of ccEe plants.

(C) Pollen grain of cue1-1/eno1-2(+/−) with a wild-type-like appearance.

(D) Close-up of the pollen grain shown in (C) with increased numbers of starch granules in the plastids and a slightly deformed and swollen intine.

(E) Pollen grain of cue1-1/eno1-2(+/−) with a wild-type-like size but affected exine and intine structures.

(F) Close-up of the pollen grain shown in (E) with a focus on the underdeveloped exine structure and the strongly deformed intine.

(G) Pollen grain of cue1-1/eno1-2(+/−) with a deformed pollen wall, high numbers of starch granules, and large vacuole-like structures.

(H) Close-up of the pollen grain shown in (G) with a focus on the impaired exine and intine structures.

(I) Pollen grain of cue1-6/eno1-2(+/−) with a wild-type-like appearance but an impaired exine structure.

(J) Close-up of the pollen grain shown in (I) with a focus on the underdeveloped exine structure.

(K) Strongly deformed pollen grain of cue1-6/eno1-2(+/−).

(L) Close-up of the pollen grain shown in (K).

Ex, exine; In, intine; L, lipid body; ER, endoplasmatic reticulum; V, vacuoles; VL, vacuole-like bodies; M, mitochondria; P, plastids; S, starch granules. Bars = 2 and 0.5 μm for the overviews and close-ups, respectively.