Figure 2.

cib1 Is Required for Proliferation in Planta.

Infected leaves were stained with Chlorazole Black E 3 DAI. The solopathogenic strain SG200 (a1 mfa2; bE1/bW2) penetrates the leaf surface via appressorium-like structures (A) and continues to proliferate in planta (B). Clamp cells of proliferating SG200 hyphae (C), indicative of active mitosis. Deletion of cib1 (D) or clp1 (G) in SG200 does not interfere with formation of appressoria, but proliferation of fungal hyphae is blocked ([E] and [H]) and clamp cells are not observed ([F] and [I]). Arrows mark appressorium-like structures ([A], [B], and [D] to [I]) or clamp cells (C). Bars = 20 μm.