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. 2010 Aug 6;22(8):2856–2871. doi: 10.1105/tpc.110.074625

Table 6.

Ratios of Kinetic Parameters of GlcT and GalT Activities

Enzyme	k_cat,GlcT/k_cat,GalT	K_m,GlcT/K_m,GalT	(k_cat/K_m)_GlcT/(k_cat/K_m)_GalT
Vv GT6-Q373H^a	0.0044	0.90 (for quercetin)	0.005 (for quercetin)
		0.36 (for UDP-sugar)	0.013 (for UDP-sugar)
Vv GT6-P19T^a	1.26	1.62 (for quercetin)	0.78 (for quercetin)
		0.53 (for UDP-sugar)	2.38 (for UDP-sugar)
Wild-type Vv GT6^b	0.70	2.57 (for quercetin)	0.27 (for quercetin)
		0.77 (for UDP-sugar)	0.93 (for UDP-sugar)
Vv GT5-R140W^c	6.85	0.91 (for quercetin)	7.39 (for quercetin)
		0.34 (for UDP-sugar)	19 (for UDP-sugar)
Vv GT5-R140W-T19P^a	2.12	2.53 (for quercetin)	0.86 (for quercetin)
		0.74 (for UDP-sugar)	2.95 (for UDP-sugar)

^a

Values for the Vv GT6-Q373H, Vv GT6-P19T, and Vv GT5-R140W-T19P mutants were calculated using the data presented in Table 5.

^b

Values for the wild-type enzyme were calculated using the data presented in Table 3.

^c

Values for the Vv GT5-R140W mutant were calculated using the data presented in Table 2.