Figure 5. The C-terminus of MCT2 plays a role in its association with endogenous basigin, but not co-expressed embigin.

(A) Rates of L-[¹⁴C]lactate (0.5 mM) uptake into oocytes expressing the MCT or MCT chimaera indicated, in the presence of absence of co-expressed embigin. Uptake was assayed at a time where uptake is linear with time: 2.5 min for all except 5 min for MCT1/2 and MCT2trn, and 10 min for MCT2/1. Results are means±S.E.M. for the number of separate oocytes shown in Table 1. (B) Immunofluorescence microscopy data of the oocytes using C-terminal antibodies against MCT2 or, in the case of the MCT2trn construct, against the HA tag inserted at the N-terminus. Emb, embigin.