Figure 3. Pcif1 regulates Pdx1 protein accumulation and Pdx1 target gene expression in primary mouse islets.

(A) Western blot of islets isolated from individual mice, probed for Pdx1. (B) Quantification of Western blots of islet protein extracts, normalized to loading control. n = 4, *P < 0.05. (C) Pdx1 and Pcif1 transcript levels in isolated mouse islets. n = 5–8, *P < 0.05, **P < 0.01 compared with wild-type. (D) Insulin transcript level in mouse islets. n = 5–8, **P < 0.01.