(A) Intraperitoneal glucose tolerance of male mice (7–8 weeks old). n = 6–9 per group; P < 0.05, Pdx1+/– versus Pcif1+/gtPdx1+/–, by ANOVA. (B) Intraperitoneal glucose tolerance of female mice (10–12 weeks old). n = 6–11 per group; P < 0.001, Pdx1+/– versus Pcif1+/gtPdx1+/– by ANOVA. (C) Insulin tolerance of male mice (8–9 weeks old). P = NS for all groups. (D) Insulin tolerance of female mice (11–13 weeks old). P = NS for all groups. (E) Acute glucose-stimulated insulin secretion calculated as AUC for 15 minutes after glucose bolus (12-week-old male mice). n = 7–12 per group, *P < 0.05, **P < 0.01. (F) Glucose-stimulated insulin secretion during static incubations of isolated islets from 6- to 8-week-old male mice. Insulin secretion values were normalized to islet insulin content and expressed as fold stimulation. n = 3–6 mice per group, *P < 0.05.