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. 2010 Sep 27;120(10):3455–3465. doi: 10.1172/JCI42528

Figure 6. Aging and Maf-mediated regulation of osteoblastogenesis and adipogenesis.

Figure 6

(A) Effect of Maf overexpression in mesenchymal cells on an aging phenotype of Maf+/– mice. Three-dimensional microcomputed tomography images and histological analysis of the bone marrow (toluidine blue staining) of Maf+/– mice transplanted with Maf-transduced or mock-infected calvarial cells. Scale bar: 200 μm (top row); 100 μm (bottom row). (B) Effect of hydrogen peroxide on Maf expression in WT and Trp53–/– calvarial osteoblasts (real-time RT-PCR analysis). (C) Effect of N-acetylcys­teine (NAC) administration on an aging phenotype of Maf+/– mice. Three-dimensional microcomputed tomography images (top row) and histology of the bone marrow (toluidine blue staining, middle and bottom rows) of mice (n = 4). Images in the middle row are shown at higher magnification in the bottom row. Scale bar: 500 μm (top and middle rows); 50 μm (bottom row). (D) Microcomputed tomography and histological analysis of WT and Maf+/– mice. (E) A model of Maf-mediated reciprocal regulation of osteoblast and adipocyte differentiation. *P < 0.05; **P < 0.01.