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. 2010 Sep 27;120(10):3466–3479. doi: 10.1172/JCI42845

Figure 8. Induction of adipose tissue lipolysis activates lipid uptake by ATMs.

Figure 8

(A) SVCs isolated from perigonadal adipose tissue of high-fat diet–induced obese mice were cultured either alone or with perigonadal adipose tissue pieces (harvested from lean animals) with or without isoproterenol treatment (10 μM) to induce lipolysis in the adipose tissue fraction. The gene expression of Adfp and Cd36 in SVCs was measured. n = 5 mice/group. Data are represented as mean ± SD. (B) The expression of the chemokine receptor Ccr2 was measured in SVCs treated as described in A. Data are represented as mean ± SD. n = 5 mice/group. (C) SVCs treated with isoproterenol cultured alone (left panel) or with adipose tissue (right panel) were stained for neutral lipid with oil red O. Lipid-containing cells are marked with arrows. Scale bars: 50 μm. (D) Percentage of lipid-containing cells among SVCs treated as described in A. n = 5 mice/group. Data are represented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; †P = 0.09. (E) The presence of lipid-laden multinucleated giant cells among SVCs cocultured with adipose tissue in the presence of isoproterenol. Scale bar: 15 μm.