Ectopic Wnt8b expression was driven by a Fezf2 promoter/regulatory element, and transient transgenic embryos were analyzed at E10.5. (A–D) Transgene expression in the forebrain and optic vesicles can be monitored by YFP immunofluorescence starting at the 8- to 11-somite stage. At around E10.5, NR progenitors express Six3 (E), Rax (G), Sox2 (I), and Vsx2 (K). Similarly to Six3 conditional mutants, transgenic embryos ectopically expressing Wnt8b in the rostral forebrain fail to express any of these NR markers (arrows in F, H, J, and L). In contrast to control embryos (M and O) and also similar to Six3 conditional–mutant embryos, the entire transgenic optic vesicle expresses Pax6 (N) and Mitf (P). Scale bars: 100 μm.