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. 2010 Sep 13;120(10):3606–3616. doi: 10.1172/JCI43304

Figure 4. c-Met inhibition abrogates angiogenesis associated with epithelial Smad2 loss.

Figure 4

(A) IF staining of CD31 (green) shows increased angiogenesis in K5.Smad2–/– tongue mucosa, which was abrogated by a c-Met inhibitor PHA66572. K14 (red) was used for counterstain. (B) IF staining shows that PHA66572 did not affect Smad2 activation, as evidenced by staining of p-Smad2 (green). Note that p-Smad2 was specifically ablated in tongue epithelial cells but not in K5.Smad2–/– stroma. K14 (red) was used for counterstain. (C) Double IF staining of p–c-Met (green) and CD31 (red) shows that the vessels in K5.Smad2–/– tongue had increased p–c-Met staining (yellow or orange), which was abrogated by PHA66572 treatment. Bright green staining surrounding the vessels represents autofluorescence of the muscles in the tongue. Scale bar: 100 μm.