Figure 3.

Identification of proteins increased in both SSc and NSF secretomes. A: Three-dimensional view displaying the intensity of fluorescence of a representative spot in two different channels and their fluorescence intensities calculated by DIGE software. The magenta lines in the bottom panels indicate the spot boundaries obtained using the pixel data from the images. The spot boundaries are used for spot volume calculations using the DeCyder software.³⁰B: Dot plot of fluorescence intensity ratio of the SSc (blue diamonds) and NSF (pink squares) secretomes, both calculated against the fluorescence values of normal secretome protein spots. The yellow dotted area indicates the spots with the highest increase in both SSc and NSF secretomes compared with the normal secretome.