Table 2.

Cell Populations in Day-14 Sponges

Sponge Type	CD31-positive pericapsular endothelial cells (mean percent area positive for CD31)	Foreign body giant cells (mean number of cells with ≥5 nuclei per area)	Ki67-positive pericapsular cells (mean percent positive cells per area)
NTD (1-35)-EGFP	2.4 ± 1.1	20 ± 8	73 ± 32
mod NTD (1-35)-EGFP	2.2 ± 0.7	35 ± 18	43 ± 7

Cell populations in the pericapsular area were quantified by the following criteria: endothelial cells by measuring the area positive for CD31; foreign body giant cells by counting the number of cells with ≥5 nuclei per cell; and proliferating cells by counting the number of cells positive for Ki67. Ten random ×40 fields were counted in each sponge section, n = 6–10 sponges per group. Results are expressed as the mean percent of total cells scored as positive ± SD. There were no statistically significant differences between the experimental and control groups.