Figure 4.

Tsc2 regulates cell-cell adhesion. A: Hanging drop assays were performed in HEK293T cells transfected with indicated vectors. Arrows highlight examples of aggregates of more than four cells 2 hours after plating. B: The number of cell aggregates per 100 cells counted after transfection of the indicated vectors was tabulated. The Western blot shows the expression of E-cadherin and the transgenes used in this experiment. C: Cell aggregation of wild-type (NRK52E) and mutant (LEF2 and ERC18M) cells in hanging drop assays. Graph indicates average number of aggregates per 100 cells counted. Arrows indicate cell aggregates. **P < 0.01.