No changes occur in ductular responses to DDC with overexpression of β-catenin, but morphological disparity among the A6-positive population is present. A: Representative photomicrographs of H&E and A6 immunofluorescence staining (green) of livers from WT and TG mice after 7, 14, and 28 days of feeding with the DDC diet. Original magnification: ×100 (H&E); ×200 (A6). 4′,6-Diamidino-2-phenylindole (blue) was used to stain nuclei in immunofluorescence images. B: Atypical hepatocytes, mostly evident in TG livers at 28 days, coexpress A6 (green) and CEBPα (red) by immunofluorescence (original magnification: ×200 (left panels); ×400 (right panels). C: Quantification of total A6-positive cells in WT and TG livers after 3, 7, 14, and 28 days of DDC feeding. No significant difference was observed at any of the time points between WT and TG livers. D: Quantification of A6-positive hepatocytes in WT and TG livers after 3, 7, 14, and 28 days of DDC feeding. More A6-positive hepatocytes were observed in TG than in WT livers at 14 and 28 days. *P < 0.01.