Figure 3.

VASP mutants recapitulate VASP siRNA phenotype of FA disruption. A: YFP-tagged retroviral constructs that express deletion or truncation VASP mutants are shown. The structure of full-length wild-type VASP is depicted on the top. B: Cells expressing individual VASP mutant were stained for vinculin (red). There is marked colocalization of vinculin with EVH2-YFP or PRRdel-YFP. Scale bars = 20 μm. C: In contrast to YFP or VASPWT controls, retroviral expression of EVH1, EVH2, or PRRdel mutant significantly increased the population of cells devoid of mature FA (*P < 0.05, analysis of variance; n = 3 independent experiments).