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. 2010 Oct;177(4):2034–2045. doi: 10.2353/ajpath.2010.100421

Figure 2.

Figure 2

Cdc7 expression in breast epithelial cell lines and tissue. A: Immunoblot analysis of whole cell extracts (WCE) prepared from asynchronously proliferating cultures of normal (HMEpC), hyperproliferating (MCF10A), and malignantly transformed (MCF7, BT549, T47D, MDAMB157, and MDAMB231) breast epithelial cells probed with antibodies against Cdc7 and actin (loading control). C: CDC7 mRNA levels relative to GAPDH (invariant control) for each cell line as determined by qRT-PCR. B and D: Photomicrographs of tissue sections of representative normal premenopausal breast (B) and high-grade (grade 3) breast cancer (D) immunohistochemically stained with antibodies to the indicated proteins (original magnification, ×400). The median labeling index (LI) is shown for each marker. Median values and interquartile ranges for the cohort were as follows: Ki-67 40% (25%−69%); Mcm2 93% (70%−100%); Cdc7 14% (9%−22%); geminin 17% (11%−25%); Plk-1 14% (9%−20%); and H3S10ph 3% (1%−4%).