Table 1.

Primers and Probes Used for Real-Time Genomic Quantitative PCR. Designed with Primer Express Software V2.0 (Applied Biosystems)

Gene	Primer sequence
TP53.1
Forward	5′-GAGGCGGGCCGATCAC-3′
Reverse	5′-CTGACTGCTCTTTTCACCCATCT-3′
Probe	5′-TGAAGTAAGGAGTTCGAGACCAGCCTGG-3′
TP53.3
Forward	5′-CTGACTGCTCTTTTCACCCATCT-3′
Reverse	5′-ACAGCATCAAATCATCCATTG-3′
Probe	5′-CAGTCCCCCTTGCCGTCCCA-3′
P16
Forward	5′-GGCTGGCTGGTCACCAGA-3′
Reverse	5′-CGCCCGCACCTCCTCTAC-3′
Probe	5′-ATGGAGCCTTCGGCTGACTGGCT-3′
P15
Forward	5′-GGAAAGAAGGGAAGAGTGTCGTT-3′
Reverse	5′-CGCGCATTCCGCAGC-3′
Probe	5′-TTTACGGCCAACGGTGGATTATCCGG-3′
P14
Forward	5′-GGTTCTCGCAGTACCATTGAA-3′
Reverse	5′-TGTTCGCCTCAGTTTCCCA-3′
Probe	5′-CCTCCCTTCACACAGCCCCTCAATC-3′
GAPDH
Forward	5′-CCCCACACACATGCACTTACC-3′
Reverse	5′-CCTAGTCCCAGGGCTTTGATT-3′
Probe	5′-TAGGAAGGACAGGCAAC-3′
ALB
Forward	5′-TGAAACATACGTTCCCAAAGAGTT-3′
Reverse	5′-CTCTCCTTCTCAGAAAGTGTGCATAT-3′
Probe	5′-TGCTGAAACATTCACCTTCCATGCA-3′
CDK4
Forward	5′-TTGCATCGTTCACCGAGATC-3′
Reverse	5′-CTGGTAGCTGTAGATTCTGGCCA-3′
Probe	5′-TGACAAGTGGTGGAACAGTCAAGCTGG-3′