Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Curr Protoc Nucleic Acid Chem. 2010 Sep;CHAPTER:Unit4.41. doi: 10.1002/0471142700.nc0441s42

Table 4.

Native-PAGE Mixture

Gel % 40% Acrylamide/bisacrylamide 19:1
(mL)		 10X TAE-Mg2+
buffer (mL)		 Deionized
water (mL)		 10% APS
(µL)		 TEMED
(µL)
6 6 4 30 500 50
8 8 4 28 500 50
10 10 4 26 500 50
12 12 4 24 500 50
14 14 4 22 500 50
16 16 4 20 500 50
18 18 4 18 500 50
20 20 4 16 500 50