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Gradient of Mobile Phase B for RP-HPLC Purification of Crude POC Materiala
| Elapse time (min) | Percentage mobile phase Bb |
|---|---|
| 0 | 5 |
| 5 | 5 |
| 30 | 30 |
| 31 | 100 |
| 35 | 100 |
| 36 | 5 |
| 40 | 5 |
Gradient conditions are based on a 0.8 mL/min flow rate using an analytical Amersham Source 5RPC ST 4.6/150 column at room temperature with a 40 minute injection cycle. Mobile phase A: 0.1 M ammonium acetate, pH 7.0. Mobile phase B: acetonitrile.
Percentage is at elapsed time.
