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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 14;184(12):6822–6832. doi: 10.4049/jimmunol.0902058

FIGURE 3.

FIGURE 3

Mice lacking two or more CD3 ζ ITAMs have an increase in cell death compared with controls. A, Thymic iNKT cells from the indicated mice were stained with anti-CD3 ε Pacific Blue, PBS57-loaded CD1d tetramer PE, annexin V allophycocyanin, and 7AAD and then analyzed by flow cytometry. Annexin V and 7AAD staining was analyzed on CD3intermediate CD1d tetramer+ iNKT cells. Numbers represent the percentage of cells in each quadrant. B, Graph indicates the percentage or absolute numbers of iNKT cells that are annexin V+ 7AAD+ using at least six mice per group pooled from three independent experiments. Statistics were generated by Kruskal–Wallis test. ***p < 0.0001; **p < 0.001. C, Proliferative responses of iNKT cells. Cells from the various mice were labeled with CSFE and stimulated with α-galactosylceramide for the indicated times. The cells were subsequently washed and analyzed by flow cytometry. Data are representative of three independent experiments.