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. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Biochemistry. 2010 Sep 21;49(37):8094–8104. doi: 10.1021/bi100879m

Table 3.

Kinetic constants of Arg248 mutants of PBP 5. WT=wild-type. The number of measurements are given in parentheses.

kcat/Km
(M−1 s−1)		 k2/KS
(M−1s−1)		 t1/2
(min)		 k3
(s−1 × 10−5)
Substrate Ac2-KAA Penicillin G Cefoxitin Penicillin G Penicillin G
WT 47.0 ± 1.9
(n=4)		 450 ± 70
(n=6)		 3410 ± 20
(n=3)		 14.9 ± 1.6
(n=2)		 78.3 ± 8.5
R248K 2.3 ± 0.9
(n=4)		 134 ± 20
(n=7)		 1760 ± 80
(n=3)		 25.9 ± 1.7
(n=3)		 44.8 ± 2.8
R248A 1.2 ± 0.5
(n=3)		 125 ± 14
(n=5)		 1550 ± 150
(n=3)		 14.3 ± 0.4
(n=3)		 80.8 ± 2.1