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. 2010 Jun 1;116(10):1787–1794. doi: 10.1182/blood-2009-10-250910

Figure 1.

Figure 1

TFPI targeting vector with 5′ DNA probe Southern blot screening. (A) Schematic representation of the introduction of the targeting vector and subsequent removal of the PGK-NEO cassette using flippase-mediated flippase recombination target site. (B) 129Sv/J embryonic stem cells were screened for successful targeting of TFPI exon 4 using Southern blot analysis that used a 5′ DNA probe differentiating wild-type and floxed alleles. (C) Germline transmission of the targeting vector was also assessed using Southern blot screening.