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Cytochrome c mitochondrial immunoactivity in HD caudate nucleus. 3D deconvolutional digital imaging of combined immunofluorescence of calbindin antisera identifying medium-sized spiny caudate neurons and cytochrome c antisera marking mitochondria from moderate Grade 2 HD and very severe Grade 4 HD patients, compared with an age-matched control subject. The loss of mitochondria confirmed the findings observed in the COX2 and SOD2 studies.
