FIG. 5.

Calcium quantification and Fourier transform infrared analysis. (A) The mineralization of the constructs was assessed by measuring the calcium deposited by cells. Both cell types deposited calcium in the osteogenic medium, indicating an osteoblast phenotype. p < 0.05 is considered significantly different (^#significantly different from the growth medium; ^*significantly different from other cell type on same surface; ^$significantly different from plate with same cell type). (B) The chemical composition of the mineral was analyzed by Fourier transform infrared spectroscopy. The annotated peaks are the signature of a carbonate-containing, poorly crystalline hydroxyapatite, indicative of physiologic mineral. The remaining peaks are due to the PCL nanofiber mesh, as seen in the acellular mesh. Color images available online at www.liebertonline.com/ten.