FIG. 6.

Calcein staining. Constructs were stained with calcein to observe the presence of the mineral deposits. (A) The osteogenic samples stained with calcein, illustrating that cells had deposited mineral uniformly on the nanofiber meshes. Images were taken at 10 × magnification. Scale bar indicates 200 μm and applies to all images. (B) The calcein staining was quantified by measuring the fluorescence using a plate reader. The data revealed greater mineralization by AFS cells. p < 0.05 is considered significantly different (*significantly greater from the MSC osteogenic medium; ^#significantly greater from growth with same cell type). Color images available online at www.liebertonline.com/ten.