FIG. 7.

Cell-seeded nanofiber meshes for in vitro delivery. (A) To investigate the use of nanofiber meshes for cell delivery, AFS cells were seeded on nanofiber meshes and wrapped around a three-dimensional (3D) collagen scaffold for 2 weeks in vitro. For comparison, cells were seeded throughout the scaffold. (B–E) Three-dimensional confocal images of the Live/Dead-stained scaffold and mesh. The projections of the 3D images are shown. The surface and top views are views of the 3D image looking from the top. The side view is the view of the 3D image looking from the side. The green box indicates the area and view being analyzed. (B) The collagen scaffold with cells seeded throughout had more cells on the exterior with numerous dead cells in the interior. (C) When a cell-seeded mesh was wrapped around the scaffold, cells migrated on to the peripheral surface of the scaffold and displayed high viability. (D) Top and cross-sectional surfaces of scaffold wrapped with cell-seeded mesh. Cells also colonized the top surface of the scaffold and migrated more than 500 μm into the scaffold from the mesh. (E) The seeded mesh was confluent with cells. Images were taken at 10 × magnification. Scale bar indicates 200 μm and applies to all images. Color images available online at www.liebertonline.com/ten.