FIG. 6.
(A) Schematic diagram of the testing chamber used to condition module sheets. Modules were partially embedded in alginate gel on top of a nylon mesh and conditioned electrically for 10 days. (B) After 10 days of continuous stimulation, macroporous RAEC/CM module sheets were removed from the alginate and nylon mesh and assessed by field stimulation. (C) ET and MCR of RAEC/CM sheets and CM-only sheets; ET was significantly higher in the presence of RAEC (**p < 0.001, n = 3). (D) Enhanced green fluorescent protein-transfected RAEC were uniformly distributed, at a relatively low density, over RAEC/CM modular sheets, 10 days after stimulation. (E) RAEC/CM sheets were reseeded with 3 × 106 RAEC, using the same protocol as in the first seeding, after removing the alginate gel. With this protocol, RAEC surface coverage was increased. Color images available online at www.liebertonline.com/ten.