FIG. 8.

(A, B) Triple-stained (vimentin, troponin I, and connexin-43) modular sheets after 10 days of stimulation indicate that surfaces of module sheets were covered by a layer of fibroblasts with an underlying layer of CM. (C, D) Connexin-43-positive structures were also observed. (E, F) Colocalization of connexin-43 and troponin I was confirmed by overlaying high magnification images, suggesting that the CM were electrically coupled. Color images available online at www.liebertonline.com/ten.