Table 1.

Medium Compositions (Presumed Key Differences) and Serum Types Used in the Study

Name	Native CM	Coculture	Seeding	Native EC
Base medium	DMEM	DMEM	MCDB-131/DMEM (50/50)	MCDB-131
Components (mM)
L-Glutamine	4	4	2	0
Magnesium sulfate	0.814	0.814	5.42	10.02
D-Glucose	25	5.56	15.28	5.56
Sodium pyruvate	0	1.28	0.5	1
HEPES	10	10	5	0
Serum concentration (%)
Bovine serum	10	10	5	0
Fetal bovine serum	0	0	5	10

CM, cardiomyocyte; DMEM, Dulbecco's modified Eagle's medium; EC, endothelial cells.