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. 2010 Apr 16;16(8):2553–2564. doi: 10.1089/ten.tea.2009.0833

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 1. — HFC morphology and proliferation potential. HFCs were plated in six-well plates at 10⁴ cells/well and cultured in DMEM with 10% FBS alone (Ctrl) or with 2 ng/mL bFGF. (A) Photomicrograph of HFCs in the presence or absence of bFGF. (B) Cells were cultured in the presence or absence of bFGF and at the indicated times trypsinized, counted, and re-plated at the same density. The cumulative cell number was plotted as mean ± SD of three independent experiments (n = 3). HFCs, hair follicle stem cells; bFGF, basic fibroblast growth factor; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; SD, standard deviation.

FIG. 1. — HFC morphology and proliferation potential. HFCs were plated in six-well plates at 10⁴ cells/well and cultured in DMEM with 10% FBS alone (Ctrl) or with 2 ng/mL bFGF. (A) Photomicrograph of HFCs in the presence or absence of bFGF. (B) Cells were cultured in the presence or absence of bFGF and at the indicated times trypsinized, counted, and re-plated at the same density. The cumulative cell number was plotted as mean ± SD of three independent experiments (n = 3). HFCs, hair follicle stem cells; bFGF, basic fibroblast growth factor; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; SD, standard deviation.