FIG. 4.
HFCs demonstrated osteogenic differentiation potential. HFCs were cultured in osteogenic differentiation medium for 2 weeks. (A) RT-PCR for osteogenic markers alkaline phosphatase (ALP), Runx2, and osteocalcin (OC). Alkaline phosphates activity of HFCs that were treated with the osteogenic (B) or control (C) medium. von Kossa staining of HFCs that were cultured in the osteogenic (D) or control (E) medium for 4 weeks. Representative images are shown from one out of three independent experiments. Color images available online at www.liebertonline.com/ten.