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. 2010 Apr 16;16(8):2553–2564. doi: 10.1089/ten.tea.2009.0833

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 6. — Derivation of SMCs from HFCs using tissue-specific promoters. (A) Schematics of lentiviral vectors encoding for ZsGreen under the control of αSMA promoter (P-αSMA) or DsRed under the P-MHC. (B) HFCs were transduced with either lentivirus and P-αSMA (ZsGreen+) or P-MHC (DsRed+) cells were sorted by flow cytometry. Fluorescence images of P-αSMA (ZsGreen+) or P-MHC (DsRed+) that were cultured in the presence or absence of bFGF (2 ng/mL). (C) Sorted cells were plated in six-well plates (10⁴ cells/well) and cultured in the presence or absence of bFGF (2 ng/mL). On day 7, the cells were trypsinized and counted, and the cell number was plotted as mean ± SD of triplicate samples in a representative experiment (n = 3). SMCs, smooth muscle cells; P-MHC, myosin heavy chain promoter. Color images available online at www.liebertonline.com/ten.

FIG. 6. — Derivation of SMCs from HFCs using tissue-specific promoters. (A) Schematics of lentiviral vectors encoding for ZsGreen under the control of αSMA promoter (P-αSMA) or DsRed under the P-MHC. (B) HFCs were transduced with either lentivirus and P-αSMA (ZsGreen+) or P-MHC (DsRed+) cells were sorted by flow cytometry. Fluorescence images of P-αSMA (ZsGreen+) or P-MHC (DsRed+) that were cultured in the presence or absence of bFGF (2 ng/mL). (C) Sorted cells were plated in six-well plates (10⁴ cells/well) and cultured in the presence or absence of bFGF (2 ng/mL). On day 7, the cells were trypsinized and counted, and the cell number was plotted as mean ± SD of triplicate samples in a representative experiment (n = 3). SMCs, smooth muscle cells; P-MHC, myosin heavy chain promoter. Color images available online at www.liebertonline.com/ten.