Examples of preBötC GlyT2-EGFP pacemaker neurons. A, Respiratory-modulated discharge in a GlyT2-EGFP pacemaker neuron current clamped at interburst Vm ≈ −60 mV. A1, Bursting activity in 10 μm CNQX bath applied. Network activity is blocked. B, B1, Another example of a respiratory-modulated discharge and bursting in a GlyT2-EGFP neuron. C, Examples of respiratory-modulated discharge and “ectopic bursts” (asterisks) after depolarizing Vm. Action potentials have been truncated.