FIG. 3.

Effects of bFGF or EGF on gene and protein expression in ESC-derived neural cells. ESC-derived neural cells were cultured for 2 weeks in B27 medium supplemented with or without either 20 ng/mL of bFGF or 20 ng/mL of EGF. (A) Significant increase in NES and NEF3 expression with both GF treatments, but significant GFAP increase in EGF treatment only. (B) Immunofluorescent staining of neural cells shows positive staining for GFAP with GF treatments, but stronger staining and more cell extensions with EGF. Scale bars indicate 200 μm. *p < 0.05 (at least three experiments) when compared with the control samples (in B27 medium without EGF or bFGF) using log-transformed one-sample t-test. bFGF, basic fibroblast growth factor; EGF, epidermal growth factor; GF, growth factor. Color images available online at www.liebertonline.com/ten.