FIG. 3.

c-Met KD does not alter MSC cell cycle, survival, or colony-forming efficiency. (a) Cell cycle: KD MSC or SCR MSC were permeabilized, stained with propidium iodide and their cell cycle status was assessed based on their DNA content. The bar graph summarizes the results of three separate experiments, showing that there was no significant difference in proliferation between KD MSC and SCR MSC. (b) Survival: KD or SCR MSC were collected, stained with Annexin V and 7AAD, and analyzed by flow cytometry. The bar graph summarizes the proportion of dead and apoptotic cells from three separate experiments, and demonstrates no significant differences. (c) CFU-F assay: MSC were plated at low density (500 cells/well of six-well plate) and the colonies were counted after 14 days of culture. The bar graph summarizes the data from two separate experiments (n = 6 for each treatment), showing that although there was a significant difference between transduced and untransduced MSC (*), there was no significant difference between KD MSC and SCR MSC as assessed using one-way ANOVA. CFU-F, colony forming unit-fibroblast; ANOVA, analysis of variance.