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. 2010 May 20;16(8):2605–2616. doi: 10.1089/ten.tea.2010.0048

FIG. 2.

FIG. 2.

Hedgehog pathway activation and adipogenic differentiation in ASCs. (A) Hedgehog signaling elements following culture with N-terminal Sonic Hedgehog (Shh-N) for 48 h by quantitative real-time polymerase chain reaction (qRT-PCR), including Ptc1, Gli1, Gli2, and Gli3. (B) Hedgehog ligand expression after Shh-N exposure for 48 h, including Shh, Ihh, and Dhh. (C–F) Adipogenic differentiation with Shh-N or cyclopamine. (C) Oil red O staining with Shh-N at day 7 adipogenic differentiation (250–750 ng/mL). (D) Oil red O staining with cyclopamine at day 7 differentiation (5–20 μM). (E) PPAR-γ expression with Shh-N at 7 days, by qRT-PCR. (F) PPAR-γ expression with cyclopamine at 7 days of differentiation. Means and significance levels are calculated relative to control expression levels, a,b,c,dp < 0.01. Color images available online at www.liebertonline.com/ten.