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. Author manuscript; available in PMC: 2011 May 20.
Published in final edited form as: Cell Host Microbe. 2010 May 20;7(5):412–419. doi: 10.1016/j.chom.2010.04.004

Figure 4.

Figure 4

Knock-down of AIM2 abrogates inflammasome activation in response to L. monocytogenes. (A) The percentage of AIM2 mRNA following shRNA knockdown in immortalized macrophages was measured by qRT-PCR. mRNA analysis represents the average of 3 independent experiments and error bars represent the standard deviation of the mean. Cell death (B) and IL-1β (C) were measured following a 6 hour infection at an MOI of 5 of Scramble or AIM2 knockdown immortalized macrophages with the indicated strains. Data are presented as the average of 3 independent experiments and the error bars represent the standard deviation of the mean. (D) Representative intracellular growth of wild type L. monocytogenes (squares) or Δlmo2473 (triangles) in Scramble (solid lines) or AIM2 knockdown (dotted lines) immortalized macrophages. *indicates these values are statistically different with a p value <0.05 using a Students’ t-test.