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. 2010 Oct 1;21(19):3293–3303. doi: 10.1091/mbc.E09-10-0879

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 3. — Ist1 localizes to centrosome and midbody during mitosis. (A–C) HA-Ist1 (green) accumulates at the microtubule-organizing center/centrosome during prophase, metaphase (arrowheads), and early anaphase (A), and at the midbody during late cytokinesis (A–C). Corresponding images show the localizations of β-tubulin (red; B) and CEP55 (red; C), and merged images are at the right. A DIC image centered on the midbody is shown in an inset in B. (D) HeLa cells were costained for endogenous Ist1 (green) and β-tubulin (red), with the merged image at the right. Boxed areas are enlarged in the panels below. Bars, 10 μm.