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. 2010 Oct 1;21(19):3396–3408. doi: 10.1091/mbc.E10-06-0512

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 3. — ESCRT-III disassembly depends on Vps4 MIT associations with MIM1 and MIM2. ESCRT-III disassembly reactions were performed with inclusion of 100 nM Vps4 (black), 100 nM Vps4^ΔMIT (red), 100 nM Vps4^ΔMIT,E233Q (green), 100 nM and 1 μM Vps4^L64D (purple, MIM1 binding surface), or 100 nM and 1 μM Vps4^I18D (blue, MIM2 binding surface).